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@#In this paper, the uncertainties of correction factors of fluconazole impurities determined by HPLC standard curve method were evaluated, and the main common factors affecting the accuracy of standard curve method were found, so as to improve the accuracy of the method.In this study, the corresponding fitting lines of fluconazole and its impurities A, B, C, D, F and I were established respectively, and the ratio of the slope of fitting lines of each impurity and its corresponding principal component was calculated as the correction factor of the impurity.Then on the basis of GUM method, the uncertainty of each impurity correction factor determined by standard curve method was evaluated according to the established uncertainty evaluation scheme of correction factor determination process.The correction factor and uncertainty of fluconazole impurities A, B, C, D, F and I were 1.068 ± 0.046, 0.102 ± 0.005, 0.0582 ± 0.0031, 1.382 ± 0.121, 0.802 ± 0.067 and 1.383 ± 0.119, respectively, and the coverage factor k was 2.Finally, the contribution rate of each uncertainty component was calculated.In the relative combined standard uncertainties urel(f) of fluconazole impurities A, B, C, D, F and I correction factors, the sum of contribution rate of slope uncertainty urel(K) of the linear equation of principal component and its impurity is more than 85%; in the slope uncertainties urel(K) of linear equation, the contribution rates of uncertainties of solution concentration in 8 of 12 data groups are more than 80%, and the contribution rates of uncertainties introduced by reference substance content in solution concentration are about 80%.It can be seen that the preparation of linear solution concentration is the most influential factor in the determination of impurity correction factor by standard curve method, followed by the linear fitting process.In the preparation process of linear solution concentration, the purity of reference substance is the most influential factor, followed by weighing and pipetting times.The conclusion can help the experimenters to better formulate experimental plans and ensure the accuracy of the results when doing similar work.
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The components of traditional Chinese medicine(TCMCs) are the basic unit of raw materials for Chinese medicines, and their physical and chemical properties directly affect the choice of dosage forms and the optimization of prescriptions. However, most of TCMCs are multi-component complex systems, and the characterization of their overall properties is still in the exploration stage. On the basis of biological activity, the representative components are determined, and then the individual characteristics are fitted with the weight coefficient of efficacy contribution rate, which may provide reference for characterizing the overall properties of TCMCs. In this study, with the pharmacological effects of isoproterenol(ISO)-induced myocardial ischemia in rats as the indicators, the pharmacodynamic contribution rates of three representative components of chishao terpene glucoside components(CSTGCs) were evaluated by the normalization weighting method. The contribution rates of paeoniflorin, paeoniflorin and benzoylpaeoniflorin were 54.87%, 32.46% and 12.67%, respectively. The oil-water partition coefficients of paeoniflorin, albiflorin, benzoylpaeoniflorin in water and buffer solutions with different pH values were measured, and the oil-water partition coefficients of CSTGCs were characterized by the weight of their pharmacodynamics contribution rate. The results showed that the apparent oil-water partition coefficient(log P) of CSTGCs in the phosphate buffer system such as n-octanol-water(pH 2.0, 2.5, 5.0, 5.8, 6.8) were 0.18-0.22, indicating that CSTGCs have common absorption and low permeability, providing basis for the preparation of CSTGCs.
Subject(s)
Animals , Rats , Coronary Artery Disease , Glucosides , Medicine, Chinese Traditional , Myocardial Ischemia , Terpenes , WaterABSTRACT
OBJECTIVES: Calcium (Ca) is an insufficiently consumed nutrient, whereas phosphorus (P) intake has exceeded the recommended intake level in Korea over the past decade. The purpose of this study was to analyze dietary Ca and P intakes and their contribution rate according to dish groups.METHODS: A 24-hour dietary recall survey of 640 healthy adults (aged 19–69 years) was undertaken twice in four Korean provinces. Dietary Ca and P intakes and their rates of contribution from 31 major dish groups were analyzed and compared by gender, age group, and region.RESULTS: The average Ca and P intakes of the subjects were 542.1 ± 222.2 mg/d and 1,068.3 ± 329.0 mg/d, respectively. The intakes of Ca and P as percentages of recommended nutrients intake (RNI%) were 71.7 ± 29.8% and 152.6 ± 47%, respectively, and the percentages under the estimated average requirement were 60.3% for Ca and 3.8% for P. The RNI% of Ca was not significantly different between males and females, but was significantly higher in subjects in the sixties age group than in other age groups and was significantly lower in the Korean capital than in other regions. The RNI% of P did not significantly differ by gender or age groups, but it was significantly higher in the capital than in Gyeong-sang. The five major dish groups contributing to Ca intake (contribution rate) were milks/dairy products 69.2 ± 109.2 mg/d (12.6%), soups 55.6 ± 69.6 mg/d (10.1%), stir-fried foods 53.1 ± 70.7 mg/d (9.7%), stews 43.4 ± 85.4 mg/d (7.9%), and kimchi 38.4 ± 31.8 mg/d (7.0%). The five major dish group contributing to P intake (contribution rate) were cooked rice 160.7 ± 107.1 mg/d (14.9%), stir-fried foods 88.5 ± 89.4 mg/d (8.2%), soups 76.7 ± 85.8 mg/d (7.1%), one-dish meals 63.3 ± 94.4 mg/d (5.9%), and stews 62.6 ± 89.3 mg/d (5.8%). The dish groups contributing to Ca and P intakes differed somewhat by gender, age group, and region.CONCLUSIONS: Programs to improve the nutritional status of Ca and P intakes should consider the differences in Ca and P contribution rates by dish groups as well as by gender, age group, and region.
Subject(s)
Adult , Female , Humans , Male , Calcium , Calcium, Dietary , Korea , Meals , Nutritional Status , PhosphorusABSTRACT
Objective To study the pollution and source apportionment differences of different periods PM2.5 in the residential community of suburb in Tianjin City during heating and non-heating periods. Methods From 2015 to 2016, daytime and nighttime PM2.5 samples were collected at a community in the suburb of Tianjin. The mass concentration of PM2.5 samples and major chemical components in PM2.5, including metal elements, polycyclic aromatic hydrocarbons (PAHs) and inorganic water-soluble ions were monitored. Positive matrix factorization (PMF) model was used to apportion potential sources of metal elements, PAHs and inorganic water-soluble ions in daytime and nighttime PM2.5. Results In the heating period, the concentrations of some metal elements of suburban residential community were higher in the daytime than in the nighttime. In the non-heating period, the concentrations of some PAHs and inorganic water-soluble ions of suburban residential community were higher in the nighttime than in the daytime. Meanwhile, the concentrations of some metal elements were greater in the daytime than in the nighttime. When in heating period, the main source of PM2.5 in the suburban residential community was coal combustion during daytime and its source contribution rate was 50.1% while secondary aerosol and fuel combustion emissions of gasoline and diesel vehicles were main sources during nighttime and their source contribution rates were 41.0% and 35.9%. The principal source of daytime PM2.5 in the suburban residential community was indoor activity emissions during non-heating period, and secondary aerosol was main source during nighttime and their source contribution rates were 29.8% and 31.1%. Conclusions The pollution status of PM2.5 in residential communities of suburban is serious, and the source apportionment of day and night PM2.5 samples has different in different heating periods.
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Objective To understand the iodine nutritional status and source distribution of residents aged 18 and above in non-water-borne high iodine areas in Tianjin, and compare the methodological difference between the 3 d dietary survey method and the 24 h urine iodine method when calculate total dietary iodine. Methods According to the Chinese adult chronic disease and nutrition surveillance program, a total of 1 634 inhabitants aged 18 and above were enrolled from Hexi, Nankai, Hongqiao, Wuqing, Jinnan, Baodi and Jizhou district of Tianjin. The total dietary iodine intake was obtained with 3 d dietary recall and condiment weighing method, And the iodine nutritional status of adult residents in Tianjin and iodine contribution rate of each part were described.403 investigators were randomly selected from the 1 634 inhabitants mentioned above for 24 h urine iodine concentration detection, and the difference between 3 d dietary survey method and urine iodine conversion method when calculate total dietary iodine were compared. Results The median dietary iodine intake of adult residents in non-water-borne high iodine areas of Tianjin was 207.13 μg/standard person day,70.3%respondents were in iodine adequate state. It was found that salt iodine is the main source of total dietary iodine (78.2%), followed by food iodine (13.2%) and water iodine (8.6%). Overall, the dietary iodine intake estimated by 3 d dietary survey method was higher about 10% compared with the result estimated by 24 h urine iodine concentration. Conclusions The adults in the non-water-borne high iodine areas in Tianjin was generally in iodine adequate state. Salt iodine is still the main source of dietary iodine, and little difference is found about the total dietary iodine intake estimated by 3 d dietary survey and 24 h urine iodine conversion method.
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Objective: By taking classical prescription of Gegen Qinlian Decoction (GQD) as the example, this research aims at studying the law of change between the clinical dosage of Chinese medicine and its effect. Methods: The test groups were divided into nine groups according to nine different dosages of GQD (1.65-28.05 g/kg). Each group was given the same dosage for six times. Then the factors of serum protein, blood sugar, insulin, glycated hemoglobins, and insulin resistance index were extracted. Results: Two common factors were extracted and the variance contribution rates were 55.601% and 24.157%, respectively. The first factor could represent blood sugar, glycated hemoglobins, and insulin resistance index, while the second factor could represent serum protein and insulin. Conclusion: Through analyzing the experimental data of GQD in the treatment of diabetes mellitus, it is found that Factor Analysis can achieve better results in eliminating the interference from human factors. Therefore, Factor Analysis is an effective and suitable method for studying the dose-effect relationship of Chinese material medica in clinical therapy.
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The purpose of this study was to investigate the influence of physiological factors which effect oxygen kinetics and energy system contribution on the power of Wingate test (WT), with focusing on the difference of aerobic capacity. Twenty three male track and field athletes (sprinters, long distance runners and decathletes) performed the WT on electromagnetic-braked cycle ergometer. The applied resistance was 7.5% of body weight, and the duration was 60 seconds. Moreover, aerobic capacity (maximal oxygen uptake [VO<sub>2</sub>max]) was determined by an incremental test, and anaerobic capacity (maximal accumulated oxygen deficit [MAOD]) was determined by a supramaximal constant load test. The oxygen uptake during each test was recorded by a breath-by-breath method. The participants were divided into two group which was high VO<sub>2</sub>max group (High group; n = 11) and low VO<sub>2</sub>max group (Low group; n = 12). In the results, although the VO<sub>2</sub>max was significantly higher in the High group, the MAOD was not significantly different between two groups. The oxygen uptake during WT was significantly higher in the High group, and the accumulated oxygen deficit during WT was significantly higher in the Low group. The aerobic contribution was significantly higher in the High group than in the Low group. In contrast, the anaerobic contribution was significantly higher in the Low group than in the High group. These results suggest that by the difference of aerobic capacity, aerobic and anaerobic energy supply contribution was different in WT.